Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhongxiu Hu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-29 08:21:59
This part is the cDNA of a mutated calcium ion channel protein.
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K2028000_sequence (Version 1) |
Description
This part encodes the gene of Calcium release-activated calcium channel protein 1 (Orai1), which is a part of calcium selective ion channel in humans. Orai1 calcium channels in the plasma membrane are activated by stromal interaction molecule-1 (STIM1), an endoplasmic reticulum calcium sensor, to mediate store-operated calcium entry (SOCE).Store-operated calcium (Ca2+) entry (SOCE) is activated in response to cellular stimuli that deplete the sarco/endoplasmic reticulum (ER) lumen of Ca2+, a prerequisite event that opens exquisitely Ca2+ selective plasma membrane (PM) channels, augmenting cytosolic Ca2+ levels and refilling ER stores. The principal molecular components of SOCE in many cell types include the ER-inserted stromal interaction molecule-1 (STIM1)3,4 and the Orai1 PM channel subunits5,6,7,8, which physically interact at ER???PM junctions in a functional Ca2+ release-activated Ca2+ (CRAC) channel complex. SOCE through CRAC channels is integral to myriad signalling pathways in electrically excitable and non-excitable cells, the most prominent being immune cells in which inheritable mutations in STIM1 and Orai1 have been linked to devastating immunodeficiency diseases.Therefore, we use this part to help with eliminating Ca2+ in hard water.