Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Max Schelski, Niklas Schmacke, Cathleen Hagemann, Sophia Mädler
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 12:07:11
pSBbs1AK1 (replicative Bacillus subtilis expression vector)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K2029015_sequence (Version 1) |
Description
This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has an kanamycin resistance for cloning in E.coli and ampicillin resistance for selection in B. subtilis.Notes
A double resistance for selection in both B.subtilis and E.coli was neededSource
pSB1A3 and a plasmid from Japan| Sequence Annotation | Location | Component / Role(s) |
| pMB1 Ori (Coli) repU Ori (subtilis) KanR AmpR pBR1 ble | 264,878 2436,3441 3600,4370 1039,1698 2012,2020 4593,4991 | sequence_feature feature/misc feature/misc sequence_feature feature/cds CDS CDS feature/cds primer_binding_site feature/primer_binding CDS feature/cds |