BBa_K2030002

BBa_K2030002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2030002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Hellgren

Date created: 2016-08-16 11:00:00

Date modified: 2016-10-19 06:45:35

Acetate kinase (ackA) from Synechocystis

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2030002_sequence (Version 1)

Description

This parts contains the coding region of Acetate kinase (AckA) + a potential native terminator (50 bp downstream of the gene) from Synechocystis sp. PCC 6803. It's involved in the Phosphate acetyltransferase-acetate kinase pathway and catalyzes the conversion of acetyl-CoA to acetate. In our project this part was overexpressed in Synechocystis to increase the acetate production.

Notes

The complete sequence of the genome of Synechocystis sp. PCC 6803(Genbank accession number NC_000911) was used to design primers for amplification of this gene.

FW primer: ATGAAATTCCTGATTCTCAATGC

RV primer: AGCCTAATTCAACATTTATCTTCAC

The reverse primer was designed to also include 50 bp downstream of the gene as a potential native terminator.

Source

Genomic DNA from Synechocystis sp. PCC 6803

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
AckA	1,1242	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2030002/1

Member of these Collections

• iGEM Parts Registry