BBa_K2032001

BBa_K2032001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2032001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chun Mun Loke
Date created: 2016-08-26 11:00:00
Date modified: 2016-10-19 11:25:25

Fructose Pathway



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K2032001_sequence (Version 1)

Description

Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.

(add detail)

Notes

Used Gibson Aseembly

Source

Methanotrophic bacteria Methylococcus capsulatus

Sequence Annotation Location Component / Role(s)
BBa_R0011
lac O1
-35
lac O1
-10
RBS-3\Weak
BBa_B0032
RBS
BBa_R0011
BBa_B0032
MDH2
BBa_K2032003
RBS-3\Weak
BBa_B0032
RBS
hps
BBa_K1585201
RBS-3\Weak
BBa_B0032
RBS
phi
BBa_K1585202
BBa_B0010
stem_loop
BBa_B0012
T7 TE
polya
stop
BBa_B0015
1,54
3,19
20,25
26,42
43,48
64,76
64,76
70,73
91,146
153,166
172,1331
85,1331
1340,1352
1340,1352
1346,1349
1359,1997
1359,1997
2006,2018
2006,2018
2012,2015
2025,2582
2025,2582
2591,2670
2602,2645
2679,2719
2686,2705
2706,2719
2712,2712
2591,2719
feature/BioBrick engineered_region
operator feature/operator
feature/promoter promoter
operator feature/operator
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/protein CDS
engineered_region feature/BioBrick
ribosome_entry_site feature/rbs
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
feature/cds CDS
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
CDS feature/cds
engineered_region feature/BioBrick
feature/BioBrick engineered_region
feature/stem_loop stem_loop
feature/BioBrick engineered_region
feature/stem_loop stem_loop
polyA_site feature/polya
feature/stop stop_codon
feature/BioBrick engineered_region
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2032001/1