BBa_K2036023

BBa_K2036023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2036023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhangyu Cheng
Date created: 2016-09-19 11:00:00
Date modified: 2016-09-24 02:03:17

RBS-iLDH-TT-pRM-RBS-beta-galactosidase



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K2036023_sequence (Version 1)

Description

patp2-RBS-CI-TT-pR-RBS-CIII

Notes

patp2 are active within pH7~9, so CI will be produced to inhibit pR thus we can regulate CIII's expression by PH level.

Source

patp2 are from Corynebacterium glutamicum ATCC 13032, CI, CIII and pR are all from bacteriaphage lambda.

Sequence Annotation Location Component / Role(s)
RBS core
Interval sequence
iLDH
Terminater
beta-galactosidase
s_mutation
s_mutation
s_mutation
s_mutation
1,15
16,21
22,1212
1213,1341
1342,1747
945,945
1450,1450
1465,1465
1489,1489
ribosome_entry_site feature/rbs
feature/misc sequence_feature
CDS feature/cds
stem_loop feature/stem_loop
feature/cds CDS
point_mutation feature/s_mutation
point_mutation feature/s_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2036023/1