BBa_K2036023

BBa_K2036023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2036023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zhangyu Cheng

Date created: 2016-09-19 11:00:00

Date modified: 2016-09-24 02:03:17

RBS-iLDH-TT-pRM-RBS-beta-galactosidase

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K2036023_sequence (Version 1)

Description

patp2-RBS-CI-TT-pR-RBS-CIII

Notes

patp2 are active within pH7~9, so CI will be produced to inhibit pR thus we can regulate CIII's expression by PH level.

Source

patp2 are from Corynebacterium glutamicum ATCC 13032, CI, CIII and pR are all from bacteriaphage lambda.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS core	1,15	ribosome_entry_site, feature/rbs
Interval sequence	16,21	feature/misc, sequence_feature
iLDH	22,1212	CDS, feature/cds
Terminater	1213,1341	stem_loop, feature/stem_loop
beta-galactosidase	1342,1747	feature/cds, CDS
s_mutation	945,945	point_mutation, feature/s_mutation
s_mutation	1450,1450	point_mutation, feature/s_mutation
s_mutation	1465,1465	feature/s_mutation, point_mutation
s_mutation	1489,1489	feature/s_mutation, point_mutation

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2036023/1

Member of these Collections

• iGEM Parts Registry