Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Toky RATOVOMANANA
Date created: 2016-09-01 11:00:00
Date modified: 2016-10-10 09:13:24
Pseudomonas resinovorans PHA synthase 1
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2042001_sequence (Version 1) |
Description
This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer.This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA (Yang et al.)
site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA); site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481
We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is not on the Serine 477 on Serine 475.
which is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1).
Notes
We used this sequence as second enzyme in order to producePLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere