BBa_K2045003

BBa_K2045003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2045003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Thiban Navaratnarajah
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 11:57:02

CDC28-Cyclin-dependent kinase catalytic subunit



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2045003_sequence (Version 1)

Description

In order to have effective cell battle with our microgladiators??? It was important to ensure that cell division of our nanoparticle attached cells didn???t occur. Cell division would not only make it difficult for cell isolation in our microfluidic battle dome, but it will also compromise the integrity of the cell???s nanoweaponry; since, the coating of nanoparticles will be halved as the cell membrane goes through cell division.
The solution was CDC28, a gene that allows users to control the growth of their cells by steadily controlled external temperature. The cells will only be active, cell division, at 24 degrees and inactive, restricted cell division, at 37 degrees. By attaching nanoparticles to our yeast cells with CDC28, and keeping them at 37 degrees in the microfluidic battle system, we can ensure an effective battle between our microgladiators without compromise of their attached nanoweapons.

Notes

CRISPR was needed to be used in order to effectively transform this useful mutant inti E.coli

Source

S.cerevisiae mutant

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2045003/1