BBa_K2048001

BBa_K2048001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2048001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Seray Cicek

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-14 11:08:59

Long GolS

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2048001_sequence (Version 1)

Description

This composite part contains Tet promoter, teto,GolS,GolB and LacZ genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. GolB is a promoter that gets activated by GolS factor. This circuit produces golS under the regulation of TetO promoter. Synthesized GolS protein binds to GolB promoter and synthesizes LacZ which generates a visible color.

Notes

Our main goal was to generate a circuit that allows production of GolS transcriptional factor and activate production of LacZ. This is achieved by placing golB before the LacZ region

Source

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Components

Access	Instance	Definition
public	BBa_K2048003	BBa_K2048003
public	BBa_K2048006	BBa_K2048006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2048003	1,580	BBa_K2048003
BBa_K2048006	581,917	BBa_K2048006

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2048001/1

Member of these Collections

• iGEM Parts Registry