Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Seray Cicek
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 11:40:58
Long GolS (P118A)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2048002_sequence (Version 1) |
Description
This composite part contains Tet promoter, teto,GolS(P118A),GolB and LacZ genes. GolS is a MerR family transcriptional regulator, gold-responsive activator of gol and ges genes. This circuit contains mutant GolS P118A which is highly sensitice to gold. GolB is a promoter that gets activated by GolS factor. This circuit produces golS under the regulation of TetO promoter. Synthesized GolS protein binds to GolB promoter and synthesizes LacZ which generates a visible color.Notes
This circuit had same design considerations as BBa_K2048001. In addition, high sensitivity was introduced.Source
Salmonella Typhimurium Mutant| Access | Instance | Definition |
| public public | BBa_K2048004 BBa_K2048006 | BBa_K2048004 BBa_K2048006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2048004 BBa_K2048006 | 1,588 589,925 | BBa_K2048004 BBa_K2048006 |