| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K2050420_sequence (Version 1)
|
Description
The green fluorescent protein (GFP) is a protein originally obtained from the jellyfish Aequorea victoria that emits green fluorescence upon exposure to light in the blue to ultraviolet range and is typically used in molecular biology and genetics as a reporter. The enhanced GFP (or EGFP) was developed to allow for its practical usage in mammalian cells.
Notes
The amino acid sequence was entered into IDT's online codon optimization tool to obtain the first DNA sequence, which was subsequently run through several rounds of manual optimization. At each round, the sequence was run through GenScript's online rare codon analysis tool to evaluate the codon optimization index (CAI), frequency of rare codons, GC content and negative cis or repeat elements. The rounds of manual optimization aims to eliminate possible bacterial or mammalian promoters, AGG codons, Chi sites, ter sites, dem sites, polyadenylation sites, Pasteurellaceae or Neisseriaceae DNA uptake sequences, K12 methylation sites, TATA boxes, palindromes and splice sites.
Source
The amino acid sequence was obtained from www.uniprot.org (accession number C5MKY7); the DNA sequence is our own.
