BBa_K2050421

BBa_K2050421 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2050421

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Muhammad Farhan Maruli

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 10:06:20

Thosea Asigna Virus 2A peptide linker (T2A)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2050421_sequence (Version 1)

Description

2A peptide is a self-cleaving peptide linker. The sequence encoding the 2A peptide is typically included between the coding sequences of two proteins without stop codons to co-express the two proteins in a single vector. The two coding sequences will be transcribed onto one mRNA molecule along with the coding sequence for the 2A peptide. During translation, however, the peptide will cleave the peptide bond between the glycine and the proline residue on its C-terminus. The result is two different polypeptide chains.

The coding sequence for 2A peptide is far shorter than that of an IRES, making it preferable in constructing a multicistronic vector. The consequence of using the 2A peptide linker, however, is that the upstream protein will have additional residues from the 2A peptide on its C-terminus and the downstream protein will start with a proline.

Notes

The amino acid sequence was optimized for human expression to achieve maximum codon adaptation index possible (evaluated with GenScript's rare codon analysis tool) with considerations to its flanking sequences.

Source

We looked up the amino acid sequence in Yang et al. (doi:10.1038/gt.2008.90) and Tang et al. (10.1523/JNEUROSCI.0359-09.2009), and then created the DNA sequence ourselves.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2050421/1

Member of these Collections

• iGEM Parts Registry