BBa_K2053000

BBa_K2053000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2053000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mathieu Hubert
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 05:16:42

T. pseudonana silaffin kinase (tpSTK1)



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K2053000_sequence (Version 1)

Description

Thalassiosira pseudonana silaffin kinase (tpSTK1) phosphorylates diatom silaffins. We propose to use it for this property. Indeed, we hope that phosphorylation of silica-binding peptide increases its biosilification properties.

Notes

Polyhistidine tag (6 His) was added at the N-terminal

Source

This part comes from Thalassiosira pseudonana and we took the sequence by using its GenBank accession number (EED92887) [Sheppard V et al, J Biol Chem. 2010 Jan 8;285(2):1166-76, doi: 10.1074/jbc.M109.039529, Epub 2009 Nov 4].

Sequence Annotation Location Component / Role(s)
His-Tag
Start codon
Stop codon
Stop codon
TEV cleavage site
tpSTK1
4,21
1,3
1573,1575
1570,1572
22,42
43,1569
feature/tag tag
start_codon feature/start
feature/stop stop_codon
stop_codon feature/stop
sequence_feature feature/misc
feature/protein CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2053000/1