Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mathieu Hubert
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 05:16:42
T. pseudonana silaffin kinase (tpSTK1)
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K2053000_sequence (Version 1) |
Description
Thalassiosira pseudonana silaffin kinase (tpSTK1) phosphorylates diatom silaffins. We propose to use it for this property. Indeed, we hope that phosphorylation of silica-binding peptide increases its biosilification properties.Notes
Polyhistidine tag (6 His) was added at the N-terminalSource
This part comes from Thalassiosira pseudonana and we took the sequence by using its GenBank accession number (EED92887) [Sheppard V et al, J Biol Chem. 2010 Jan 8;285(2):1166-76, doi: 10.1074/jbc.M109.039529, Epub 2009 Nov 4].| Sequence Annotation | Location | Component / Role(s) |
| His-Tag Start codon Stop codon Stop codon TEV cleavage site tpSTK1 | 4,21 1,3 1573,1575 1570,1572 22,42 43,1569 | feature/tag tag start_codon feature/start feature/stop stop_codon stop_codon feature/stop sequence_feature feature/misc feature/protein CDS |