Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Uriel Eleazar Barboza P??rez
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-20 10:14:33
Promoter chiA74 from Bacillus thuringiensis
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K2057000_sequence (Version 1) |
Description
The promoter chiA74 control the expression of the endochitinase chiA74 from a strain of Bacillus thuringiensis. In spite of is derived from a gene of a Gram positive bacterium, it is funcional in a Gram negative bacteria, and control the expression of the same gene in Escherichia coli in a constitutive form. This promoter can be to control the expression constitutively of a gene in E. coli.Notes
The only modification was that we included two artificial restriction sites in the in the 5??and 3??terminus, so that we could clone it in a vector.Source
Promoter chiA74 is found on the chromosome of a mexican strain of Bacillus thuringiensis subsp. kenyae (Gen bank accession number AF424979)| Sequence Annotation | Location | Component / Role(s) |
| -35 promoter -10 promoter | 379,384 399,404 | promoter feature/promoter promoter feature/promoter |