BBa_K2057004

BBa_K2057004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2057004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jos?? Eleazar Barboza Corona
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-17 09:14:52

LasR under the regulation of the promoter BtI-BtII



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K2057004_sequence (Version 1)

    Description

    The promoter BtII from Bacillus thuringiensis regulate the expression of the cry genes during sporulation, but it is functional in Escherichia coli in a constitutive form.

    Notes

    As promoter is not available in the biobricks, we amplified, sequenced and characterized using the cry1Ac gene as template.

    Source

    Promoter BtI-BtII is harbor in a megaplasmid of B. thuringiensis, lasR was obtained from a biobrick.

    Access Instance Definition
    public
    public
    		BBa_K2057001
    BBa_C0079
    		BBa_K2057001
    lasr

    Sequence Annotation Location Component / Role(s)
    BBa_K2057001
    BBa_C0079
    		1,387
    394,1174
    		BBa_K2057001
    lasr

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2057004/1
     

    Attachments

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