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BBa_K2060000

BBa_K2060000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2060000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cardiff_Wales Team, Geraint Parry

Date created: 2016-09-22 11:00:00

Date modified: 2016-09-30 06:06:30

CRISPR-Cas9 guide RNA targeting to 16S RNA

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K2060000_sequence (Version 1)

Description

This part includes a guide RNA that will target Cas9 to the E.Coli 16S ribosomal RNA locus. The guide RNA is expressed with the Cas9-recognised RNA scaffold. Expression of the guideRNA is driven by the T7 promotor with Lac0 binding site and followed by a T7 terminator sequence.

Notes

Used standard design considerations for guide RNAs

Source

E.coli 16S ribosomal RNA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7 LacO KpnI guideRNA T7term guideRNA	1,17 20,44 45,50 51,152 153,188 51,53	feature/promoter promoter feature/misc sequence_feature feature/scar feature/dna sequence_feature sequence_feature feature/misc start_codon feature/start