BBa_K206030

BBa_K206030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K206030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Amelia Hardjasa and Alex Ng

Date created: 2009-05-10 11:00:00

Date modified: 2015-05-08 01:11:24

Lock 1

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_K206030_sequence (Version 1)

Description

crR12-loop-RBS sequence taken from Isaacs et al. (1), with modifications to remove an extra EcoRI site. crR12 binds to the RBS, preventing translation of the mRNA unless a matching "key" RNA is also present in the cell.

1) Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.

Notes

Sequence provided in the supplementary table of Isaacs paper is lacking 5 bases present in the diagram that appear to contribute to secondary structure folding. These bases were included in the sequence, with one A->T change to avoid the inclusion of an EcoRI site.

Source

Taken from Isaacs et al., with modifications to remove an extra EcoRI site.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
crR12	7,25	feature/binding, non_covalent_binding_site
loop	26,31	feature/stem_loop, stem_loop
rbs	37,44	feature/rbs, ribosome_entry_site

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K206030/1

Member of these Collections

• iGEM Parts Registry