Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Ng
Date created: 2009-06-05 11:00:00
Date modified: 2015-05-08 01:11:24
Key 1
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K206031_sequence (Version 1) |
Description
A standardized BioBrick part of "Key 1" from Issacs' taR12 sequence (1). This part "unlocks" the "Lock 1" crR12 sequence (See Part:BBa_K206001). To activate a lock, assemble a promoter immediately upstream of the coding strand of this part. [New and experimental:] To deactivate this key, assemble a reverse promoter immediately downstream of the non-coding strand of this part.Notes
taR12 sequence from Isaacs et al. (1) with the following modifications:a. removal of last 6 nucleotides from the 3' end, to reduce probability of unwanted binding from complimentary scars
b. changing of nucleotide 13 from G to U, to allow complementarity with the suffix scar to block an exposed rbs
Source
1) Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.| Sequence Annotation | Location | Component / Role(s) |
| Loop YUNR recognition | 38,41 3,6 | feature/stem_loop stem_loop feature/misc sequence_feature |