Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: avi matityahu
Date created: 2016-08-22 11:00:00
Date modified: 2016-10-10 04:39:15
gRNA1 for editing F508 deletion
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K2061002_sequence (Version 1) |
Description
This plasmid contains the sequence coding for the gRNA for editing the CFTR F508 deletion under U6 promoter. The part was cloned into the pSB1C3 plasmid with chloramphenicol rresistance.Notes
the part cloned between EcoRI and PstI sites.Source
synthetic production of the part| Sequence Annotation | Location | Component / Role(s) |
| EcoRI site PstI site U6 Ex11 gRNA1 PAM gRNA scaffold seq | 1,6 466,471 7,324 326,345 346,348 349,425 | sequence_feature feature/misc feature/misc sequence_feature promoter feature/promoter CDS feature/cds sequence_feature feature/misc CDS feature/cds |