Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Almog Angel
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-17 02:36:45
Heat Labile Toxin B subunit with DNA binding Domain
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2061005_sequence (Version 1) |
Description
A new chimeric protein that include both DNA Binding Domain (DBD) and Heat Labile Toxin B (LTB) connected via a linker.LTB is an enterotoxin from E.coli, not toxic.
LTB can used as a homing device, since it can bind, with high affinity (KD 10-10 ??? 10-12 M), to the various ganglioside molecules present on the cell membrane of all epithelial cells.
DBD, wass originally taken from a virus, has the ability to bind DNA.
LTB+DBD can used as a delivery system, in vivo, for DNA into Epithelial cell tissue.
Notes
LTB and DBD have to include in betwwen a liner of ~20 amino acid long.Source
LTB comes from E.coliDBD comes from AV1 virus