BBa_K2064001

BBa_K2064001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2064001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dragos S. Chiriac
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-21 04:33:18

TyrA Donor COM



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K2064001_sequence (Version 1)

Description

We developed a BioBrick that is used to link the inserted protein with a protein containing a Tyrocidine B acceptor domain. This allows the two proteins to come together in physical space through protein-protein interactions and increase the efficiency of enzymatic gene clusters. It is under the control of an inducible T7 promoter and terminator pair from a pET-16b vector allowing expression of protein. It contains a golden gate cloning site for the scarless insertion of NRPS modules or other proteins.

Notes

Xba1 cut site removed from the region downstream of the promoter

Source

Synthetic, codon optimized for E. coli

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2064001/1