| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K2064002_sequence (Version 1)
|
Description
We developed a BioBrick that is used to link the inserted protein with a protein containing a Tyrocidine B donor domain as well as a Tyrocidine C acceptor domain. This allows the two proteins to come together in physical space through protein-protein interactions and increase the efficiency of enzymatic gene clusters. It is under the control of an inducible T7 promoter and terminator pair from a pET-16b vector allowing expression of protein. It contains a golden gate cloning site for the scarless insertion of NRPS modules or other proteins
Notes
Xba1 cut site removed, originally located downstream of the promoter
Source
Synthetic, codon optimized for E. coli
