BBa_K2065001

BBa_K2065001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2065001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tom van Sonsbeek

Date created: 2016-09-25 11:00:00

Date modified: 2016-10-18 02:49:37

CT52-mNeonGreen

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2065001_sequence (Version 1)

Description

CT52 is linked to mNeonGreen by a flexible GGS-10 linker to make sure the mNeonGreen is able to move towards other bound proteins. This insert is placed into a pET28a vector and
expressed by the E. Coli BL21 (DE3) bacteria.
The part is used for BRET assays in the tetrameric scaffold together with the CT52-SsNL and CT52-LsNL. mNeonGreen-CT52 will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm.
The sequence of our CT52-mNeonGreen has been verified by Starseq. It contains the prefix and suffix with
the correct restriction sites (EcoRI, XbaI, SpeI and PstI). mNeonGreen-CT52 is 956 bp long.

Notes

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Source

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Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2065001/1

Member of these Collections

• iGEM Parts Registry