Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom van Sonsbeek
Date created: 2016-09-25 11:00:00
Date modified: 2016-10-18 02:49:37
CT52-mNeonGreen
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2065001_sequence (Version 1) |
Description
CT52 is linked to mNeonGreen by a flexible GGS-10 linker to make sure the mNeonGreen is able to move towards other bound proteins. This insert is placed into a pET28a vector andexpressed by the E. Coli BL21 (DE3) bacteria.
The part is used for BRET assays in the tetrameric scaffold together with the CT52-SsNL and CT52-LsNL. mNeonGreen-CT52 will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm.
The sequence of our CT52-mNeonGreen has been verified by Starseq. It contains the prefix and suffix with
the correct restriction sites (EcoRI, XbaI, SpeI and PstI). mNeonGreen-CT52 is 956 bp long.