Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-07-11 11:00:00
Date modified: 2016-10-12 11:59:59
UNS Standard Adapter with Red Chromoprotein.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2066001_sequence (Version 1) |
Description
This part serves as an adapter to the UNS standard adopted by the WM iGEM 2016 team. The part expresses a red chromoprotein as an indicator molecule and teams can perform PCR to amplify the backbone using a UNS 2 reverse and UNS 3 forward primer. This will allow you to go into a gibson assembly with another fragment containing USN 2 and 3 at either end.The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
Notes
We wanted to maintain a standard that allowed us an easy and consistent way to PCR and gibson clone our samples, which we did not find in the Prefix and Suffix. PCRs and Gibson assemblies using USN 2 and 3 primers have been very effective.Source
The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. Other sequences are from the iGEM registry.| Access | Instance | Definition |
| public public public public public public | BBa_K2066018 BBa_J23110 BBa_B0034 BBa_K592012 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_J23110 BBa_B0034 eforRed BBa_B0015 BBa_K2066019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2066018 BBa_J23110 BBa_B0034 BBa_K592012 BBa_B0015 BBa_K2066019 | 1,40 41,75 76,87 88,768 769,897 898,937 | BBa_K2066018 BBa_J23110 BBa_B0034 eforRed BBa_B0015 BBa_K2066019 |