Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-08-31 11:00:00
Date modified: 2016-10-12 12:37:31
plLac0-1 sfGFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2066026_sequence (Version 1) |
Description
This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship of IPTG-sfGFP without RiboJ.Notes
Part designed to be compared with K2066014 (with RiboJ) to see the effect of RiboJ on the transfer function.Source
The promoter, RBS, and reporter sequences from Lou et. al 2012 "Ribozyme-based insulator parts buffer synthetic circuits from genetic context". UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_K2066508 BBa_K2066528 BBa_K2066510 BBa_K2066509 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_K2066508 BBa_K2066528 BBa_K2066510 BBa_K2066509 BBa_B0015 BBa_K2066019 |