Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-28 09:19:26
pBAD - LacI/Mcherry fusion
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K2066028_sequence (Version 1) |
Description
This is a Mcherry reporter protein fused to a functional LacI repressor under the control of the arabinose inducible promoter pBAD on the UNS backbone. This part was originally designed to be used as an upstream circuit simulator for WM16_iGEM's circuit control toolbox. The inducible pBad represents an arbitrary circuit with LacI as the product, and as the input to the orthogonal circuit used to modify the transfer function. The mcherry allows for the quantification of the input in the same cell as the quantification of the output (GFP).Notes
Codons were optimized for E. Coli expression and non compatible cut-sites were removed.Source
LacI, linker, and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.).| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_I0500 BBa_B0034 BBa_K2066512 BBa_K2066513 BBa_B0015 BBa_K2066019 | BBa_K2066018 pBad/araC BBa_B0034 BBa_K2066512 BBa_K2066513 BBa_B0015 BBa_K2066019 |