Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-08-30 11:00:00
Date modified: 2016-10-12 01:37:21
pTET GFP + TetR on UNS
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2066053_sequence (Version 1) |
Description
This part is allow pTET GFP on UNS (K2066023) and TetR on UNS (K2066022) to be on the same plasmid. By allowing these two parts on the same plasmid, it will reduce stress on a cell when doing multiple plasmid transformations. In this case of our project, we needed to put the repressor (TetR), promoter it binds to (pTET) and a TetO binding array in the same cell. Because we combined the repressor and promoter onto one plasmid, we only transformed with two plasmids.Notes
Made this part to make multiple plasmid transformations more effective.Source
This part was derived mostly from iGEM registry parts. It is a combination of K2066022 and K2066023. It uses UNS sequences from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public | BBa_K2066018 BBa_R0040 BBa_K2066529 BBa_B0034 BBa_K2066530 BBa_E0040 BBa_K2066529 BBa_B0015 BBa_K2066507 BBa_J23105 BBa_K2066529 BBa_B0034 BBa_K2066530 BBa_C0040 BBa_K2066529 BBa_B0015 BBa_K2066019 | BBa_K2066018 p(tetR) BBa_K2066529 BBa_B0034 BBa_K2066530 GFP BBa_K2066529 BBa_B0015 BBa_K2066507 BBa_J23105 BBa_K2066529 BBa_B0034 BBa_K2066530 tetR BBa_K2066529 BBa_B0015 BBa_K2066019 |