| Types | DnaRegion
|
| Roles | DNA
sequence_feature
|
| Sequences | BBa_K2066525_sequence (Version 1)
|
Description
This is an intermediate part used in the construction of WM16 LacO ICA parts (WM16_K2066011-WM16_K2066013). This part contains the LacO repeat sequence used by WM for ICA creation of LacO binding arrays. Lac Operator Sequence based off of Addgene Plasmid #17655. ICA design method based off of Briggs, A. W., Rios, X., Chari, R., Yang, L., Zhang, F., Mali, P., & Church, G. M. (2012). Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic acids research, gks624.
Notes
N/A
Source
Lac Operator Sequence based off of Addgene Plasmid #17655. ICA design method based off of Briggs, A. W., Rios, X., Chari, R., Yang, L., Zhang, F., Mali, P., & Church, G. M. (2012). Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic acids research, gks624.
