BBa_K2071969

BBa_K2071969 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2071969
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pavle Jeremic
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-28 05:53:34

GLD1 is a general reductase, it is characterized in https://microbialcellfactories.biomedcentral.com



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2071969_sequence (Version 1)

Description

This part codes for a reductase that can reduce erythrose to erythritol. It depends on NADPH as a redox co-factor.

Notes

We had two major design considerations when working with this sequence. Firstly we had to ensure that the sequence was codon optimized for eventual expression in Bacillus subtilis. We then also had to ensure that no RFC10 restriction sites were in the submitted sequence. We were able to correct both of these issues simultaneously using IDT???s codon optimization software.

Source

This part was originally isolated from the genome of Trichoderma reesei as the gene GLD1.
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0458-y

igem#experience None  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K2071969/1