Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pavle Jeremic
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-28 05:53:34
GLD1 is a general reductase, it is characterized in https://microbialcellfactories.biomedcentral.com
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2071969_sequence (Version 1) |
Description
This part codes for a reductase that can reduce erythrose to erythritol. It depends on NADPH as a redox co-factor.Notes
We had two major design considerations when working with this sequence. Firstly we had to ensure that the sequence was codon optimized for eventual expression in Bacillus subtilis. We then also had to ensure that no RFC10 restriction sites were in the submitted sequence. We were able to correct both of these issues simultaneously using IDT???s codon optimization software.Source
This part was originally isolated from the genome of Trichoderma reesei as the gene GLD1.https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0458-y