Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ching song
Date created: 2016-10-03 11:00:00
Date modified: 2016-10-05 03:37:03
pBAD-driven synthetic beta-glucosidase gene with araC
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2072000_sequence (Version 1) |
Description
Express araC and pBAD-driven beta-glucosidase gene.Notes
The enzyme is to be used in solid fermentation of notoginseng root powder for ginsenosides bio-availability improvement.Source
The beta-glucosidase gene is a truncated form of a natural gene from Microbacterium esteraromaticum strain GS514 (GenBank: JN603821.1) with optimized codons according to E.coli codon bias. The rest sequence is from E.coli K12 genome.| Sequence Annotation | Location | Component / Role(s) |
| terminator BBa_J61048 truncated glycoside hydrolase from Microbacterium esteraromaticum strain GS514 codon optimzed for E.coli expression araC pBAD/araC promoter pBAD/araC promoter terminator BBa_B0015 | 1,113 114,1799 2138,3016 1800,2137 1800,2137 3017,3145 | sequence_feature feature/misc feature/cds CDS feature/cds CDS feature/promoter promoter feature/promoter promoter sequence_feature feature/misc |