Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danielle Bauhan, Alexander Lacrampe, Joseph Lee, and Richard Anthony
Date created: 2016-08-08 11:00:00
Date modified: 2016-08-13 06:42:07
pSB416-GPD: URA3-selectable S. cerevisiae Expression Vector (RFC 10)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K2077000_sequence (Version 1) |
Description
This is a RFC10 standard yeast expression vector adapted from the widely used plasmid p416-GPD. The MCS of p416-GPD was replaced with the standard BioBrick prefix and suffix and an illegal PstI restriction site near the URA3 promoter of p416-GPD was removed. In the resultant plasmid, pSB416-GPD (5743 bp), the BioBrick prefix and suffix is situated between the yeast GPD promoter and CYC1 terminator allowing for facile cloning and expression of translational units in yeast. M13 primers flank the transcriptional unit.The plasmid has a ColE1 origin and ampicillin resistance for replication in E.coli. It also contains a yeast CEN/ARS providing high fidelity maintenance and low copy number in yeast.
Notes
Replacing the MCS with standard BioBrick prefix and suffix, removing illegal restriction sites and avoiding deleterious sequences in the promoter and terminator regions.Source
This plasmid is adapted to the BioBrick Standard from p416-GPD (Mumberg, D., M??ller, R. and M. Funk (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 156. pp. 119???22.)| Sequence Annotation | Location | Component / Role(s) |
| BB suffix CYC1 Terminator URA3 CDS F1 ORI URA3 Promoter CEN6 Centromere bla promoter bla CDS ColE1 ORI GPD Promoter BB prefix | 1,21 23,270 1065,1868 478,933 1869,2080 2343,2864 2883,2987 2988,3848 4019,4607 5073,5716 5722,5743 | sequence_feature feature/misc sequence_feature feature/misc feature/cds CDS feature/misc sequence_feature feature/promoter promoter feature/misc sequence_feature promoter feature/promoter CDS feature/cds sequence_feature feature/misc promoter feature/promoter feature/misc sequence_feature |