Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Holly Cox
Date created: 2016-08-14 11:00:00
Date modified: 2016-08-16 01:10:45
pSB413-GPD: HIS3-selectable S. cerevisiae Expression Vector (RFC 10)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K2077001_sequence (Version 1) |
Description
This is a RFC10 standard yeast expression vector adapted from the widely used plasmid pSB413-GPD. The MCS of p413-GPD was replaced with the standard BioBrick prefix and suffix and an illegal PstI restriction site near the HIS3 promoter of p413-GPD was removed. In the resultant plasmid, pSB413-GPD (5828 bp), the BioBrick prefix and suffix is situated between the strong constitutive yeast GPD promoter (TDH3) and CYC1 terminator allowing for facile cloning and expression of translational units in yeast. Standard VF2 and VR primer binding sites are included and M13 primer binding sites bracket the promoter and terminator.The plasmid has a ColE1 origin and ampicillin resistance for replication in E. coli. It also contains a yeast CEN/ARS and HIS3-selectable marker to provide high fidelity maintenance and low copy number in S. cerevisiae.
Notes
TBASource
TBA| Sequence Annotation | Location | Component / Role(s) |
| BioBrick Suffix CYC1 Terminator F1 ORI HIS3 HIS3 Promoter CEN 6 Centromere bla promoter Bla CDS ColEI ORI GPD Promoter BioBrick Prefix | 1,21 23,270 478,933 1193,1852 1853,2039 2425,2928 2965,3069 3070,3930 4101,4689 5158,5801 5807,5828 | feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS promoter feature/promoter sequence_feature feature/misc feature/promoter promoter feature/cds CDS feature/misc sequence_feature feature/promoter promoter feature/misc sequence_feature |