Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xintong Xia, Xander Lacrampe, Richard Anthony
Date created: 2016-08-16 11:00:00
Date modified: 2016-10-14 11:21:51
mls-yeGFP
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K2077002_sequence (Version 1) |
Description
According to 2015 Rose-Hulman iGEM team, the coding sequence of mitochondrial ribosomal protein mRPS12 starts with a mitochondrial localization signal (mls). From previous research, it is possible that this mls protein is able to transport and direct any attached protein into the mitochondria. To verify the hypothesis brought up in 2015, we constructed a hybrid system with the mls protein and a reporter protein. Instead of using the regular GFP, yeast-enhanced green fluorescent protein (yeGFP) was used in this system as optimal gene expression can be achieved.Notes
yeast codon optimized GFP is used instead of regular GFP. And a GS linker was used to create this mls-yeGFP hybrid system.Source
TBA| Sequence Annotation | Location | Component / Role(s) |
| RBS(WT mRPS12 Kozak) mRPS12 mls GS Linker yeGFP | 1,22 19,87 88,120 121,837 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |