Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-09-21 11:00:00
Date modified: 2016-09-22 07:37:24
bax-sgRNA1
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K2080001_sequence (Version 1) |
Description
This guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.Notes
We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.Source
Form Bba_K2080001| Sequence Annotation | Location | Component / Role(s) |
| U6 promoter gRNA for Bax promoter and Bax-1 promoter gRNA scaffold | 1,264 265,284 285,367 | feature/promoter promoter sequence_feature feature/misc feature/misc sequence_feature |