BBa_K2080008

BBa_K2080008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2080008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 05:10:58

bax-sgRNA4



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K2080008_sequence (Version 1)

Description

This guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.
Sequence and Features

Notes

We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.

Source

From BBa_K2080000

Sequence Annotation Location Component / Role(s)
U6 promoter
1,265
feature/promoter promoter
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2080008/1