BBa_K2083001

BBa_K2083001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2083001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Virginia Massa

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 10:10:45

APOBEC 2XTEN

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2083001_sequence (Version 1)

Description

Coding region for APOBEC1, an enzyme that performs C to U edits on DNA and RNA with a 2xXTEN (88 nucleotide) linker. The one extension linker sequence is TCTGGCTCAGAAACG-CCCGGTACGTCCGAGTCTGCAACACCAGAAAGC. The 2xXTEN is the one extension sequence twice. APOBEC with a 1xXTEN is BBa_K2083000, and the 3xXTEN can be found here BBa_K2083002.
Source:
Komor AC et al.Nature. Epub ahead of print (2016).

Notes

We preformed codon optimization to get rid of any EcoRI, XbaI, SpeI, and PstI sites.

Source

This part comes from the sequence of the enzyme in rats.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2083001/1

Member of these Collections

• iGEM Parts Registry