BBa_K2083012

BBa_K2083012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2083012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Virginia Massa

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 03:54:16

Wild Type Human Beta Globin Fused to GFP Reporter

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K2083012_sequence (Version 1)

Description

Coding region for human beta globin with GFP reporter fused to the end. GFP fused to beta globin with a premature termination codon (PTC) can be found at BBa K2083011.

The validation of the β-globin reporters followed the same procedures as the ATG/ACG reporters. The wild type β-globin reporter showed a much lower level of expression compared to the ATG reporter (14.77% of positive control).


http://2016.igem.org/wiki/images/8/85/T--WPI_Worcester--globineGFP_data_.png

Figure 1: Efficiency of WT/PTC β-globin reporters compared to positive control. Error bars represent standard error between normalized results from 3 biological replicates of the experiment.

Notes

We performed codon optimization to removed any illegal restriction sites.

Source

This a human beta globin sequence with the first two exons fused. GFP is fused to the end of the end of the sequence.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2083012/1

Member of these Collections

• iGEM Parts Registry