Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nikhil Nair
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 06:28:26
CRISPR/Cas9 sgRNA Targeting Locus 401 on the DD96 Human Gene
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K2087002_sequence (Version 1) |
Description
This is the transcript for the sgRNA that hybridizes with S. pyogenes Cas9 in order to guide the endonuclease to make a double stranded break at a sequence complementary to the RNA. The transcription is under a the control of a T7 promoter, and targets the 401 locus on the human DD96 gene. This particular sgRNA is created from the BBa_K1818000 biobrick, which is an empty sgRNA cassette with two adjacent Bbs1 cut sites where the target spacer should go. This part was created by digesting the empty cassette with Bbs1 and ligating the 20bp spacer to reform the plasmid.Notes
No design considerations.Source
The T7 cassette comes from the BBa_K1818000 biobrick. The sequence for the spacer is complementary to the human DD96 gene.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1818000 T7 Promoter Target 401 Spacer sgRNA | 1,121 1,15 16,32 33,121 | feature/BioBrick engineered_region promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature |