Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yuxin He
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-14 05:51:25
pbaB: a 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain.
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2088007_sequence (Version 1) |
Description
PbaB is a [2Fe-2S]-type ferredoxin, located in Sphingobium wenxiniae JZ-1.It can express in Ecoli（DH）5ɑ or BL21 in plasmid pPBA.It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions.
Mostly, pbaB Implement function with pbaA1A2. PbaA1A2B are the oxygenase and ferredoxin components of the angular dioxygenase (3-phenoxybenzoate 1???,2???-dioxygenase) responsible for the angular dioxygenation at the 1??? and 2??? positions on the benzene moiety of 3-phenoxybenzoate and that the ferredoxin PbaB is indispensable for the angular dioxygenase
Notes
We have searched the function of pbaB ,and the relationship between pbaA1A2.Source
The number in genebank is KJ009324.1【link https://www.ncbi.nlm.nih.gov/protein/633267949】And it comes from Sphingobium wenxiniae JZ-1.
reference: A Novel Angular Dioxygenase Gene Cluster Encoding 3-Phenoxybenzoate 1=,2=-Dioxygenase in Sphingobium wenxiniae JZ-1,
Chenghong Wang, Qing Chen, Rui Wang, Chao Shi, Xin Yan, Jian He, Qing Hong, Shunpeng Li
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon TAG stop codon | 1,3 319,321 | start_codon feature/start stop_codon feature/stop |