Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xia Chen
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-18 04:16:07
nativeRBS-C23O with double Terminator
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_K2088009_sequence (Version 1) |
Description
This composite part includes a constitutive promoter (BBa_J23100), a catechol 2,3-dioxygenase gene with native rbs (BBa_K2088008) and the double terminator (BBa_B0015).This device can be used as a reporter, because catechol 2,3-dioxygenase (C23O) can convert catechol to bright yellow product 2-hydroxymuconate semialdehyde.
Notes
Uses J23100, a strong Anderson Promoter, to produce high levels of expression of C23O.Source
This is a compostie part including catechol 2,3-dioxygenase gene from Sphingobium sp. YBL2 encoded in part BBa_K2088008.| Access | Instance | Definition |
| public public | BBa_K2088008 BBa_B0015 | BBa_K2088008 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2088008 BBa_B0015 | 1,1021 1030,1158 | BBa_K2088008 BBa_B0015 |