T

BBa_K2088666 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2088666
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shi Kejian
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-08 03:01:00

MhbT is responsible for the uptake of 3-hydroxybenzoate



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2088666_sequence (Version 1)

Description

MhbT is a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.By sequence comparison, MhbT exhibited 42% identity with the 4-hydroxybenzoate and protocatechuate transporter PcaK from P.putida, 27% identity with the benzoate transporter BenK from Acinetobacter sp. Using the MhbT-GFP fusion proteins , we can find that MhbT is located to the periphery of cells. It can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA.

Notes

None

Source

We get the part from the bacteria M5a1, and the sequence of the gene was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/AY648560.1

Sequence Annotation Location Component / Role(s)
ATG start codon
TAA stop codon
MhbT coding region
1,3
1357,1359
1,1359
feature/start start_codon
stop_codon feature/stop
CDS feature/cds
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2088666/1