BBa_K2091001

BBa_K2091001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2091001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ben Vaknin
Date created: 2016-09-17 11:00:00
Date modified: 2016-09-19 08:55:28

Protocatechuate 3,4-dioxygenase Beta Subunit



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2091001_sequence (Version 1)

Description

Protocatechuate 3,4-dioxygenase catalyzes the convertion of 3,4-dihydroxybenzoic acid and O2 to 3-carboxy-cis,cis-muconate. Dioxygenases are oxydoreductase enzymes. They utilize a dioxygen (O2) molecule as an oxidant. As opposed to monooxygenases, in dioxygenases reactions both atoms from O2 are incorporated into the substrate. Protocatechuate dioxygenase from P. putida has a molecular weight of 200,000 daltons and contains 4 alpha subunits of 23,000 daltons, 4 beta subunits of 26,500 daltons, and 4 ferric irons, suggesting that the enzyme is a tetramer of (𝛂𝛃Fe3+) catalytic units.

Notes

None.

Source

Genomic DNA of Pseudomonas putida strain KT2440.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2091001/1