BBa_K2092005

BBa_K2092005 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K2092005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hui Wen Chong
Date created: 2016-10-01 11:00:00
Date modified: 2016-10-09 04:34:33

alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K2092005_sequence (Version 1)

Description

This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA (PalcA) and aldA promoters.

AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].


Reference:

[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385???396.

Notes

- Codon optimized the sequence retrieved from the NCBI database.
- Calculated the RBS strength by using the online tool SalisLab RBS Calculator.

Source

Aspergillus nidulans, sequence found through

igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2092005/1