Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hui Wen Chong
Date created: 2016-10-01 11:00:00
Date modified: 2016-10-02 05:07:51
Pcon (J23110) + RBS (B0034) + alcR
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2092008_sequence (Version 1) |
Description
This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA (PalcA) and aldA promoters.AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].
A constitutive promoter and strong RBS Part BBa_J34801 has been added upstream of the start codon.
Usage and Biology
This part is useful to measure the promoter strength of alcA/aldA promoters.
Reference
[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385???396.
Notes
Calculated the RBS strength by using the online tool SalisLab RBS Calculator.Source
Aspergillus nidulans. The protein was first extracted and characterized by Felenbok et al. (1988) [1]. This version is codon optimized for E.coli by Thermo Fisher Scientific and synthesized by IDT.| Access | Instance | Definition |
| public public | BBa_J23110 BBa_K2092005 | BBa_J23110 BBa_K2092005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_K2092005 | 1,35 44,2656 | BBa_J23110 BBa_K2092005 |