Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Liu iGEM
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-15 08:33:29
pLIP (SORLIPx2)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K2095000_sequence (Version 1) |
Description
This basic part consists of a synthesised truncated version of the promotor of the light-inducible protein gene (LIP) in Dunaliella microalgae. Activation of the promoter has shown to be stimulated by high intensity light when introduced into Chlamydomonas Reinhardtii (1)(2).In 2013, Park S. et al. investigated the promoter of the highly inducible promoter gene (LIP) of the marine algae Dunaliella sp.(1). They discovered several light-responsive motifs by experimenting with truncations of the promotor fused with a Renilla luciferase gene introduced in C. reinhardtii. In a more recent study from 2016, they identified sequences over-represented in light-inducible promoters (SORLIP)(2). Duplication of the SORLIP sequence upstream a 100 bp truncation of the LIP promoter showed promising results in activity when stimulated by high- and medium-intensity light. This BioBrick consists of the 2xSORLIP promotor sequence followed by an intron, optimized for use C. reinhardtii.
1. Park S, Lee Y, Lee J-H, Jin E. Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii. Planta [Internet]. 2013 Dec [cited 2016 Sep 4];238(6):1147???56. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24043576
2. Baek K, Lee Y, Nam O, Park S, Sim SJ, Jin E. Introducing Dunaliella LIP promoter containing light-inducible motifs improves transgenic expression in Chlamydomonas reinhardtii. Biotechnol J. 2016;11(3):384???92.