Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Brandes
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 09:55:39
pENTR pTRE
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K2100004_sequence (Version 1) |
Description
description here!Notes
This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.Source
This is a synthetic promoter.| Sequence Annotation | Location | Component / Role(s) |
| minCMV tetO binding site tetO binding site tetO binding site tetO binding site tetO binding site tetO binding site | 253,312 77,91 150,164 222,236 44,58 116,130 187,201 | feature/promoter promoter non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |