Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Brandes
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 09:57:26
pENTR pPRE4
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K2100009_sequence (Version 1) |
Description
description here.Notes
This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.Source
This is a synthetic promoter from IDT.| Sequence Annotation | Location | Component / Role(s) |
| minCMV PRE site PRE site PRE site PRE site | 184,241 50,65 121,136 86,101 157,172 | promoter feature/promoter feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding |