BBa_K2100010

BBa_K2100010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2100010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kathleen Brandes

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-10 09:45:48

pENTR pHybrid

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K2100010_sequence (Version 1)

Description

description here.

Notes

This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

This is a synthetic promoter from IDT.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
minCMV	234,292	promoter, feature/promoter
PRE site	64,79	non_covalent_binding_site, feature/binding
PRE site	100,115	non_covalent_binding_site, feature/binding
PRE site	136,151	feature/binding, non_covalent_binding_site
PRE site	173,188	feature/binding, non_covalent_binding_site
PRE site	209,224	feature/binding, non_covalent_binding_site
ERE site	47,60	feature/binding, non_covalent_binding_site
ERE site	84,97	non_covalent_binding_site, feature/binding
ERE site	119,132	non_covalent_binding_site, feature/binding
ERE site	156,169	non_covalent_binding_site, feature/binding
ERE site	193,206	feature/binding, non_covalent_binding_site

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2100010/1

Member of these Collections

• iGEM Parts Registry