BBa_K2100051

BBa_K2100051 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2100051
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wangui Mbuguiro
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 04:46:33

Sensor 20A



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K2100051_sequence (Version 1)

Description

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Notes

Synthesized by Jeremy Gam of MIT's Weiss Lab.

Source

human genome

Sequence Annotation Location Component / Role(s)
miRNA-20A target site x4
mKate
hEF1A
hEF1A
EBFP
5,92
10268,10972
9030,10203
1461,2634
2690,3409
sequence_feature feature/misc
feature/protein CDS
promoter feature/promoter
promoter feature/promoter
feature/protein CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2100051/1