BBa_K2100054

BBa_K2100054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2100054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Goupil

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-24 04:55:05

Sensor 18A

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K2100054_sequence (Version 1)

Description

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Notes

Courtesy of Jeremy Gam of MIT's Weiss Lab

Source

Human genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
miR-18A target site	1,92	sequence_feature, feature/misc
hEF1A	1463,2636	promoter, feature/promoter
eBFP	2692,3411	feature/protein, CDS
hEF1A	9032,10205	feature/promoter, promoter
mKate	10270,10974	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2100054/1

Member of these Collections

• iGEM Parts Registry