BBa_K2100060

BBa_K2100060 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2100060

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Goupil

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-27 10:50:26

Sensor 21-5p

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K2100060_sequence (Version 1)

Description

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Notes

Courtesy of Jeremy Gam of MIT's Weiss Lab

Source

Human genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
miR-21-5p target site	1,88	sequence_feature, feature/misc
hEF1A	1465,2638	promoter, feature/promoter
eBFP	2694,3413	feature/protein, CDS
hEF1A	9034,10207	feature/promoter, promoter
mKate	10272,10976	CDS, feature/protein

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2100060/1

Member of these Collections

• iGEM Parts Registry